Hoefer TE22 Instrukcja Użytkownika

user manualum TE22-IM/Rev.G0/07-12Hoefer TE22Tank transfer unit

• p2SpecificationsGel size up to four 9 × 10 cm gelsMax. wattage 50 WMax. voltage 100 VMax. amperage 500 mAMax. temperature 45 °CBuffer required

• p3Included but not shown:• Gel cassettes (4)• Foam sponges, 6 mm thick (4)• Foam sponges, 3 mm thick (8)• Blotting paper, sheets (25)coverelectrode

• p4Operating instructionsPerform the transfer as soon as possible after electrophoresis to minimize band diffusion. Each step is described below. Pre

• p5Prepare two lengths of 9 mm (3/8") vinyl or silicone tubing. Slide hose clamps (4 total) onto each end of two lengths of tubing. Attach one e

• p6sponge, and then place the membrane on the blot-ting paper. Place the gel—which contains a sample that has been electrophoretically separated and

• p7Install the cassette(s)1If transferring only one or two gels, choose the cassette positions nearest the center. The cassettes must be oriented so

• p8Final assembly and transfer1Install the lidThe cassettes are color coded to match the leads in the lid. To transfer toward the anode, orient the l

• p94Set the power supplyConstant current mode is recommended. If constant voltage mode is selected, carefully monitor the current (increased current

• p10Care and maintenanceCleaning• Do not autoclave or heat any part above 45 °C.• Do not expose to alcohols or organic solvents!*• Never use abrasiv

• p11Troubleshootingproblem solutionIncomplete transfer Blank areas on Remove all trapped air pockets in the transfer stack assembly: the membr

• piContents Important Information ... iiWaste Electrical and Electronic Equipment (WEEE) ...

• p12problem solutionDiffuse band patterns Transfer immediately after electrophoretic separation. If equilibrat-ing before the transfer, shorten or

• p13Electrotransfer notesElectrophoretic transfer advantagesElectrophoretic transfer of proteins and nucleic acids is much faster than the blotting m

• p14Factors affecting the transferParameters such as sample characteristics, membrane type, gel pore size, and the transfer buffer used all contribut

• p15Instrument guidelinesCoolingConsiderable Joule heat is generated during any transfer because of the high current employed, so active cooling is r

• p16Protein transfersStudy summariesGershoni and Palade (1982) investigated factors affecting protein recovery from SDS gels to nitrocellulose or DBM

• p17Protein transfer buffersUse a buffer with low ionic strength, such as the two listed below, to prevent overheating. Use the alternate CAPS buffer

• p18Nucleic acid transfersNucleic acids must normally be transferred in denatured form for most efficient binding. RNA is normally denatured with gly

• p19EDTA solutiona(0.5 M EDTA, pH 8.0, 100 ml)Na2EDTA·2H2O (FW 372.2) 0.5 M 18.6 gDissolve in 70 ml distilled water. Adjust to pH 8.0 with 10 M NaO

• p20BibliographyAlwine, J.C., Kemp, D.J., and Stark G.R., Method for detection of specific RNAs in agarose gels by trans-fer to DBM paper and hybridi

• p21Lin, W., and Kasamatsu, H., On the electrotrans-fer of polypeptides from gels to nitrocellulose membranes. Anal. Biochem. 128, 302–311 (1983).Mat

• piiImportant Information – English• If this equipment is used in a manner not speci-fied by Hoefer, Inc. the protection provided by the equipment m

• p22Ordering informationproduct quantity code no.Hoefer TE22 Tank Transfer Unit. 1 TE22 Includes: 4 gel cassettes, 8 foam sponges, 3-mm thi

• p23Hoefer, Inc. 84 October Hill Road Holliston, MA 01746Toll Free: 1-800-227-4750 Phone: 1-508-893-8999 Fax: 1-508-893-0176 E-mail: support@hoeferin

• piiiBelangrijke Informatie – Dutch• Indien deze uitrusting in een manier wordt gebruikt die niet door Hoefer, Inc. is gespecificeerd de bescherming

• pivl’instrument. Les dissolvants organiques causeront des dommages irréparables à l’unité!• Ne pas fonctionner avec les températures de tampon au-d

• pv• Sikkerheten lokket må være på plass før forbinding kraftforsyningene blyene til en kraftforsyning. • Vender all kraftforsyningsstyring av og f

• pvipor Hoefer, Inc. puede ser utilizado para operar, para mantener, y para atender a este producto.• Sólo utiliza una alimentación que es CE marcó

• pviiEnglishFrenchGermanItalianSpanishSwedishThis symbol indicates that the waste of electrical and electronic equipment must not be disposed as unso

• p1Transfer Electrophoresis Unit function and descriptionThe Hoefer® TE22 Tank Transfer unit rapidly transfers proteins, DNA, or RNA from up to four