Hoefer HE-PLUS System Instrukcja Użytkownika Pobierz pdf (Strona 22)

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p12

Solutions

Tris Acetate EDTA Buffer (TAE)

1X Working Concentration:

40 mM Tris base

20 mM Glacial Acetic Acid (NaOAc)

2.0 mM EDTA

pH 8.3

10X Stock Solution:

48.4 g Tris Base

16.4 g or 11.42 ml NaOAc

7.4 g EDTA or 20 ml 0.5 M EDTA (pH 8.0)

H

2

O to 1 liter

Tris Borate EDTA Buffer (TBE)

1X Working Concentration:

89 mM Tris Base

89 mM Boric Acid

2.0 mM EDTA

pH 8.0

10X Stock Solution:

108 g Tris Base

55 g Boric Acid

6.72 g EDTA or 40 ml 0.5 M EDTA (pH 8.0)

H

2

O to 1 liter

Sample Loading Buffer, DNA

6X Stock Solution:

30% Glycerol in H

2

O

0.25% Xylene cyanol

0.25% Bromophenol blue

pH 8

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